Enzyme-Linked Immunosorbent Assay Competitive Binding Inhibition
نویسندگان
چکیده
منابع مشابه
Vitellogenin assay by enzyme-linked immunosorbent assay (ELISA) as a biomarker of endocrine disruptor chemicals (EDCs) pollution
There are increasing evidences that show many xenobiotic chemicals (called as endocrine disruptorchemicals EDCs) through interfering with endocrine system, have the capability to induce developmentaland reproductive abnormalities in humans and animals. The yolk protein precursor vitellogenin (VTG) hasproved to be a simple and sensitive biomarker for assessing exposure of fish to EDCs especially...
متن کاملStandardization of an Enzyme-Linked Immunosorbent Assay for Detection of Infectious Bronchitis Virus Antibody.
An indirect enzyme–linked immunosorbent assay (ELISA) was developed for screening of antibody to avian infectious bronchitis virus (IBV). Antigen was prepared from whole-purified IBV Massachusetts serotype (BR 801 strain). Optimum dilution with minimum background for antigen concentration, rabbit anti-chicken conjugate and sera in developed ELISA was determined 0.1μg/ml, 1:3000 and 1:100, respe...
متن کاملEvaluation of Immune Status to Measles in Vaccinated Population in Tehran, by Enzyme-linked Immunosorbent Assay and the Hemagglutination Inhibition Techniques (1386-1387)
Background and Aims: Measles remains one of the leading causes of childhood morbidity and mortality in developing countries and is still a major public health concern in developed countries. Although live attenuated vaccine is used throughout the world, out breaks of disease still occur in many countries including Iran. Methods: The present study was performed to evaluate the immune status aga...
متن کاملEvaluation of immune status to measles in vaccinated population in Tehran, using enzyme-linked immunosorbent assay and the hemagglutination inhibition techniques
Abstract: Measles remains one of the leading causes of childhood morbidity and mortality in developing countries and is still a major public health concern in developed countries. Although live attenuated vaccine is used throughout the world, out breaks of disease still occur in many countries including Iran. Understanding measles outbreaks that occur after the initiation of measles eliminati...
متن کاملEndotoxin contamination of enzyme conjugates used in enzyme-linked immunosorbent assays.
The specificity of the enzyme-linked immunosorbent assay(s) is thought to depend on the specificity of the antibody used in the assay system. Therefore, the association of broadly reactive antigens like endotoxin with enzyme conjugates or other enzyme-linked immunosorbent assay reagents has the potential of altering the specificity of reactions in the enzyme-linked immunosorbent assay. Using th...
متن کامل